Biosensor, consisting of physico-chemical transducer, bioactive component and its thread-shaped carrier.The thread-shaped carrier enables to measure the amount of bioactive component in the units of length and cut pieces of bioactive component containing carrier, which amount of bioactive component is measured in units of length. The bioactive component and its amount are variable and the carrier is changable in biosensor. Biosensor can contain several different carriers with different bioactive components, but also carrier without biological component. Method of making biosensor, characterized in this that the thread-shaped carrier, containing bioactive component is coiled in the form of winding stairs around the physico-chemical transducer. This form of the bioactive component containing carrier enables to vary the amount of carrier and biological component; but also to measure the amount of carrier and bioactive component in the units of length.