Oleme seadnud eesmärgiks luua terapeutiliste märklaudade identifitseerimise kahekomponentne süsteem. Selleks kasutame esiteks Tandem Affiinsuspuhastamise meetodit valgukoplekside identifitseerimiseks. Selleks konstrueerime vastvad ekspressioonivektorid. Interakteeruvad valgud identifitseeritakse mass-spektomeetrilise analüüsi abil. Teiseks, konstrueerime kaksikhübriidse süsteemi bimolekulaarsete interaktsioonide identifitseerimiseks. Interaktsioone plaanime tuvastada fluorestsentsi resonantsi energia ülekande abil.
We plan to develop efficient system for identification of therapeutic targets in the human and animal cells. The system will have two basic components. First, we shall have a Tandem Affinity Purification method for identification of protein complexes, which will have a set of different vectors for expression of TAP/tagged proteins. The partners interacting with the TAP tagged protein will be identified using mass/spectrometry. The second component of this system will be two/hybrid system for animal cells, where interaction between two proteins in the specific cell compartment will be identified by Fluorescence Resonance Energy Transfer.